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Characterization of hDPSCs. A Light microscopy images of primary hDPSCs on 9th day. B Image of hDPSCs colony forming units at 14 days. C Alizarin red staining of mineralized nodules. D Oil red staining of adipogenic induction. E Alcian bale staining of chondrogenic induction. F Flow cytometric analysis of hDPSCs using multiple markers. hDPSCs were positive for CD73, CD90, and CD105, and negative for CD34 and CD45. G Positive immunofluorescence to nestin and <t>stro-1,</t> and negative to cytokeratin-3 of hDPSCs
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Characterization of hDPSCs. A Light microscopy images of primary hDPSCs on 9th day. B Image of hDPSCs colony forming units at 14 days. C Alizarin red staining of mineralized nodules. D Oil red staining of adipogenic induction. E Alcian bale staining of chondrogenic induction. F Flow cytometric analysis of hDPSCs using multiple markers. hDPSCs were positive for CD73, CD90, and CD105, and negative for CD34 and CD45. G Positive immunofluorescence to nestin and <t>stro-1,</t> and negative to cytokeratin-3 of hDPSCs
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Characterization of hDPSCs. A Light microscopy images of primary hDPSCs on 9th day. B Image of hDPSCs colony forming units at 14 days. C Alizarin red staining of mineralized nodules. D Oil red staining of adipogenic induction. E Alcian bale staining of chondrogenic induction. F Flow cytometric analysis of hDPSCs using multiple markers. hDPSCs were positive for CD73, CD90, and CD105, and negative for CD34 and CD45. G Positive immunofluorescence to nestin and <t>stro-1,</t> and negative to cytokeratin-3 of hDPSCs
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Characterization of hDPSCs. A Light microscopy images of primary hDPSCs on 9th day. B Image of hDPSCs colony forming units at 14 days. C Alizarin red staining of mineralized nodules. D Oil red staining of adipogenic induction. E Alcian bale staining of chondrogenic induction. F Flow cytometric analysis of hDPSCs using multiple markers. hDPSCs were positive for CD73, CD90, and CD105, and negative for CD34 and CD45. G Positive immunofluorescence to nestin and <t>stro-1,</t> and negative to cytokeratin-3 of hDPSCs
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anti stro 1 antibody  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology anti stro 1 antibody
Characterization of hDPSCs. A Light microscopy images of primary hDPSCs on 9th day. B Image of hDPSCs colony forming units at 14 days. C Alizarin red staining of mineralized nodules. D Oil red staining of adipogenic induction. E Alcian bale staining of chondrogenic induction. F Flow cytometric analysis of hDPSCs using multiple markers. hDPSCs were positive for CD73, CD90, and CD105, and negative for CD34 and CD45. G Positive immunofluorescence to nestin and <t>stro-1,</t> and negative to cytokeratin-3 of hDPSCs
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Characterization of hDPSCs. A Light microscopy images of primary hDPSCs on 9th day. B Image of hDPSCs colony forming units at 14 days. C Alizarin red staining of mineralized nodules. D Oil red staining of adipogenic induction. E Alcian bale staining of chondrogenic induction. F Flow cytometric analysis of hDPSCs using multiple markers. hDPSCs were positive for CD73, CD90, and CD105, and negative for CD34 and CD45. G Positive immunofluorescence to nestin and stro-1, and negative to cytokeratin-3 of hDPSCs

Journal: BMC Oral Health

Article Title: Salvianolic acid B functionalized injectable GelMA hydrogel for pulpitis in a vital pulp therapy: a dual anti-inflammatory property and enhanced reparative dentinogenesis activity material

doi: 10.1186/s12903-026-07880-z

Figure Lengend Snippet: Characterization of hDPSCs. A Light microscopy images of primary hDPSCs on 9th day. B Image of hDPSCs colony forming units at 14 days. C Alizarin red staining of mineralized nodules. D Oil red staining of adipogenic induction. E Alcian bale staining of chondrogenic induction. F Flow cytometric analysis of hDPSCs using multiple markers. hDPSCs were positive for CD73, CD90, and CD105, and negative for CD34 and CD45. G Positive immunofluorescence to nestin and stro-1, and negative to cytokeratin-3 of hDPSCs

Article Snippet: Primary antibodies included rabbit anti-human nestin (Affinity Biosciences, China), mouse anti-human stro−1 (R&D systems, USA) and mouse anti-human cytokeratin (Abcam, USA).

Techniques: Light Microscopy, Staining, Immunofluorescence